This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FN1 / Fibronectin Antibody (Biotin) LS-C60153
catalog :
LS-C60153
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
product information
AntibodyID :
60623
AntibodyName :
LS-C60153
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
FN1 / Fibronectin Antibody (Biotin) LS-C60153
Specificity :
Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
ClonalityDesc :
Polyclonal
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.
PurificationDesc :
Affinity chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in 100 µL of deionized water.
IsotypeName :
IgG
Gene :
FN1 / Fibronectin
StandardGeneSymbol :
FN1
Reactivity :
Mouse, Bovine, Rat, Human, Monkey
Usage :
ELISA (1:5000 - 1:20000), IHC (1:50 - 1:200), IP (1:100), WB (1:500 - 1:5000)
ShortWebDescription :
Fibronectin antibody LS-C60153 is a biotin-conjugated rabbit polyclonal antibody to Fibronectin (FN1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
UsageText :
Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.