This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Laminin Antibody (Biotin) LS-C60152
catalog :
LS-C60152
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
product information
AntibodyID :
60622
AntibodyName :
LS-C60152
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Laminin Antibody (Biotin) LS-C60152
Specificity :
Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
ClonalityDesc :
Polyclonal
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenDesc :
Non-reduced 400 kDa human laminin.  Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system.
PurificationDesc :
Affinity chromatography
RecommendedStorageDesc :
Store at 4°C.
ResuspensionLiquidDesc :
Reconstitute in 100 µL of deionized water.
IsotypeName :
IgG
Gene :
Laminin
Reactivity :
Human
Usage :
ELISA (1:20000 - 1:100000), IHC (1:1000 - 1:5000), IP (1:100), WB (1:2000 - 1:10000)
ShortWebDescription :
Laminin antibody LS-C60152 is a biotin-conjugated rabbit polyclonal antibody to human Laminin. Validated for ELISA, IHC, IP and WB.
UsageText :
Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Laminin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:8000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.