This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
GFP Antibody (TRITC) LS-C60054
catalog :
LS-C60054
clonality :
polyclonal
host :
domestic goat
conjugate :
TRITC
application :
western blot, immunocytochemistry
product information
AntibodyID :
60524
AntibodyName :
LS-C60054
TargetSpecies :
Aequorea victoria
Host Species :
Goat
Product Name :
GFP Antibody (TRITC) LS-C60054
Specificity :
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
ClonalityDesc :
Polyclonal
AntibodyModification :
TRITC
PresentationDesc :
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
PurificationDesc :
Affinity chromatography
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
ResuspensionLiquidDesc :
Reconstitute in 1 mL of deionized water.
IsotypeName :
IgG
Gene :
GFP
StandardGeneSymbol :
GFP
Reactivity :
Aequorea victoria
Usage :
FLISA (1:20000), IF (1:500 - 1:2500), WB (1:10000)
ShortWebDescription :
GFP antibody LS-C60054 is a TRITC-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB.
UsageText :
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP. Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Peroxidase conjugated anti-GFP assayed by immunoblot shows a 42 kD band when reacted with GFP on a western blot. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
GFP
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.