This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
MIF Antibody LS-C59678
catalog :
LS-C59678
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
60148
AntibodyName :
LS-C59678
TargetSpecies :
Human
Host Species :
Chicken
Product Name :
MIF Antibody LS-C59678
Specificity :
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
PurificationDesc :
Selective precipitation and salt fractionation followed by daialysis.
RecommendedStorageDesc :
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
ResuspensionLiquidDesc :
Reconstitute in 500 µL of deionized water.
IsotypeName :
IgY
Gene :
MIF
StandardGeneSymbol :
MIF
Reactivity :
Human
Usage :
ELISA (1:1000 - 1:5000), WB (1:500 - 1:2000)
ShortWebDescription :
MIF antibody LS-C59678 is an unconjugated chicken polyclonal antibody to human MIF. Validated for ELISA and WB.
UsageText :
This IgY fraction antibody of anti-Human macrophage migration inhibitory factor (MIF) has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry and flow cytometry. The antibody recognizes 12300 MW mature human MIF. The MIF gene encodes a protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown.
Synonyms :
MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.