CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C59514 | LifeSpan Biosciences LS-C59514 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C59514

catalog :

LS-C59514

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

WA-1

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

LifeSpan Biosciences product webpage

citations: 5

Reference
Joshi U, Chen Y, Kalemkerian G, Adil M, Kraut M, Sarkar F. Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Cancer Gene Ther. 1998;5:183-91 pubmed
Kagawa S, Fujiwara T, Hizuta A, Yasuda T, Zhang W, Roth J, et al. p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Oncogene. 1997;15:1903-9 pubmed
Yeh C, Shatkin A. A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21. J Biol Chem. 1995;270:15815-20 pubmed
Waldman T, Kinzler K, Vogelstein B. p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Cancer Res. 1995;55:5187-90 pubmed
Yang Z, Perkins N, Ohno T, Nabel E, Nabel G. The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Nat Med. 1995;1:1052-6 pubmed

product information

AntibodyID :

59984

AntibodyName :

LS-C59514

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C59514

ClonalityDesc :

Monoclonal

CloneName :

WA-1

AntibodyModification :

Unconjugated

PresentationDesc :

0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide

ImmunogenType :

Recombinant protein

ImmunogenDesc :

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

PurificationDesc :

Protein A purified

RecommendedStorageDesc :

Short term: -20°C; Long term: -20°C.

IsotypeName :

IgG1,k

Gene :

CDKN1A / WAF1 / p21

StandardGeneSymbol :

CDKN1A

Reactivity :

Human

Usage :

ELISA (1:5000 - 1:20000), IF (1:250), IHC, IHC-Fr, IHC-P, IP (1:100), WB (1:2000 - 1:10000)

ShortWebDescription :

P21 antibody LS-C59514 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for ELISA, IF, IHC, IP and WB. Cited in 5 publications.

UsageText :

This antibody is suitable for immunohistochemistry, ELISA and immunoblotting. The antibody detects a 21 kD band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21 WAF1 and not p21 complexes with other proteins. High level expression of p21 WAF1 requires induction of wild-type p53 expression (e. g. via exposure to DNA damaging agents).

Synonyms :

CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.