This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
TNF Alpha Antibody LS-C59380
catalog :
LS-C59380
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
product information
AntibodyID :
59850
AntibodyName :
LS-C59380
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
TNF Alpha Antibody LS-C59380
Specificity :
The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Antiserum
ImmunogenType :
Recombinant protein
ImmunogenDesc :
The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Short term: -20°C; Long term: -20°C.
Gene :
TNF Alpha
StandardGeneSymbol :
TNF
gene family :
TNF
Reactivity :
Human, Primate
Usage :
ELISA (1:1000 - 1:5000), IF (1:100), IHC (1:100 - 1:500), WB (1:500 - 1:2000)
ShortWebDescription :
TNF Alpha antibody LS-C59380 is an unconjugated rabbit polyclonal antibody to TNF Alpha from human. It is reactive with human and primate. Validated for ELISA, IF, IHC and WB.
UsageText :
This antiserum against Human TNFa has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17000 MW TNFa. Reactivity in other immunoassays is unknown.
Synonyms :
TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.