This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
SARS-CoV 3CL Protease Antibody LS-C59249
catalog :
LS-C59249
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
SARS coronavirus
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
59719
AntibodyName :
LS-C59249
TargetSpecies :
Coronavirus SARS-CoV
Host Species :
Rabbit
Product Name :
SARS-CoV 3CL Protease Antibody LS-C59249
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Native protein
ImmunogenDesc :
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease.  Lifesensors Inc. (www.lifesensors.com) prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
ResuspensionLiquidDesc :
Reconstitute in 100 µL of deionized water.
IsotypeName :
IgG
Gene :
SARS-CoV 3CL Protease
Reactivity :
Virus, Coronavirus SARS-CoV
Usage :
ELISA (1:10000 - 1:50000), IF, WB (1:2000 - 1:10000)
ShortWebDescription :
SARS-CoV 3CL Protease antibody LS-C59249 is an unconjugated rabbit polyclonal antibody to SARS-CoV 3CL Protease from coronavirus sars-cov. It is reactive with coronavirus sars-cov and virus. Validated for ELISA, IF and WB.
UsageText :
This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kD in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.