This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
VCAM1 / CD106 Antibody LS-C5801
catalog :
LS-C5801
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
product information
AntibodyID :
6542
AntibodyName :
LS-C5801
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
VCAM1 / CD106 Antibody LS-C5801
Specificity :
Reacts with human VCAM-1.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide, 0.1% BSA
ImmunogenType :
Recombinant protein
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG1
Gene :
VCAM1 / CD106
StandardGeneSymbol :
VCAM1
gene family :
ICAM
Reactivity :
Human
Usage :
Flo (1:10), IHC, IHC-Fr, IP
ShortWebDescription :
CD106 antibody LS-C5801 is an unconjugated mouse monoclonal antibody to human CD106 (VCAM1). Validated for Flow, IHC and IP.
UsageText :
Useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation. Flow Cytometry: 1:10. Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100U/ml) or LPS (1 ug/ml) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody.
Synonyms :
VCAM1, CD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.