This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Phosphotyrosine Antibody (clone PY20) LS-C57995
catalog :
LS-C57995
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PY20
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
58465
AntibodyName :
LS-C57995
TargetSpecies :
Synthetic
Host Species :
Mouse
Product Name :
Phosphotyrosine Antibody (clone PY20) LS-C57995
Specificity :
Recognizes phosphotyrosine, enabling the detection, characterization and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyze the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
ClonalityDesc :
Monoclonal
CloneName :
PY20
AntibodyModification :
Unconjugated
PresentationDesc :
BBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG2b
Gene :
Phosphotyrosine
Reactivity :
Synthetic
Usage :
ELISA, IF, WB (2 µg/ml)
ShortWebDescription :
Phosphotyrosine antibody LS-C57995 is an unconjugated mouse monoclonal antibody to synthetic Phosphotyrosine. Validated for ELISA, IF and WB.
UsageText :
Western Blot: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1 mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.