This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Hepatitis B x Antigen (HBxAg) Antibody (clone 3F6-G10) LS-C57338
catalog :
LS-C57338
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3F6-G10
application :
immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
57808
AntibodyName :
LS-C57338
TargetSpecies :
Virus
Host Species :
Mouse
Product Name :
Hepatitis B x Antigen (HBxAg) Antibody (clone 3F6-G10) LS-C57338
Specificity :
recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.
ClonalityDesc :
Monoclonal
CloneName :
3F6-G10
AntibodyModification :
Unconjugated
PresentationDesc :
0.09% Sodium Azide, 0.1% BSA
ImmunogenType :
Fusion protein
ImmunogenDesc :
HB-Xag-Protein A Fusion protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG2a
Gene :
Hepatitis B x Antigen (HBxAg)
Reactivity :
Virus
Usage :
ELISA, IHC-Fr (1:100), IHC-P (1:100)
ShortWebDescription :
Hepatitis B x Antigen (HBxAg) antibody LS-C57338 is an unconjugated mouse monoclonal antibody to virus Hepatitis B x Antigen (HBxAg). Validated for ELISA and IHC.
UsageText :
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.