product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
GFP Antibody (HRP) LS-C50850
catalog :
LS-C50850
clonality :
polyclonal
host :
domestic goat
conjugate :
HRP
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Gorinski N, Wojciechowski D, Guseva D, Abdel Galil D, Mueller F, Wirth A, et al. DHHC7-mediated palmitoylation of the accessory protein barttin critically regulates the functions of ClC-K chloride channels. J Biol Chem. 2020;295:5970-5983 pubmed publisher
product information
AntibodyID :
51121
AntibodyName :
LS-C50850
TargetSpecies :
Aequorea victoria
Host Species :
Goat
Product Name :
GFP Antibody (HRP) LS-C50850
Specificity :
Recognizes wild type, recombinant and enhanced GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins.
ClonalityDesc :
Polyclonal
AntibodyModification :
HRP
PresentationDesc :
10 mg/ml BSA (IgG, Protease free), PBS, pH 7.2, 0.01% Gentamicin
ImmunogenType :
Fusion protein
ImmunogenDesc :
GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG
Gene :
GFP
StandardGeneSymbol :
GFP
Reactivity :
Aequorea victoria
Usage :
ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:3000), WB (1:1000 - 1:5000)
ShortWebDescription :
GFP antibody LS-C50850 is an HRP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 2 publications.
UsageText :
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000-1:50000. Sandwich or capture. Recognizes wild type, recombinant and enhanced forms of GFP. Immunohistochemistry: 1:500-1:3000. Immunofluorescence: Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic. (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Western Blot: 1:1000-1:5000. Detects a 42kD band. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
GFP
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.