This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
GFP Antibody LS-C50832
catalog :
LS-C50832
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
51103
AntibodyName :
LS-C50832
TargetSpecies :
Aequorea victoria
Host Species :
Rabbit
Product Name :
GFP Antibody LS-C50832
Specificity :
Recognizes Green Fluorescent Protein (GFP). Species cross-reactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP. Detects 5 ng of recombinant GFP (ECL) with 1 minute exposure or less.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.2, 0.01% Sodium Azide
ImmunogenDesc :
Highly purified recombinant GFP made in E. coli.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
GFP
StandardGeneSymbol :
GFP
Reactivity :
Aequorea victoria
Usage :
ICC (1:200 - 1:1000), IF, IHC, IHC-P (1:2500 - 1:10000), IP, WB (1:2500 - 1:10000)
ShortWebDescription :
GFP antibody LS-C50832 is an unconjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ICC, IF, IHC, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunogold Electron Microscopy. Western Blot: 1:2500-1:10000 in 1X BLOTTO or 3% BSA in PBS. Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins. Immunocytochemistry: 1:200-1:1000 in 4% normal serum. Very good signal on recombinant YES-GFP chimeras expressed in COS cells. Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin. Immunohistochemistry: 1:200 for paraffin sections. Requires heat mediated antigen retrieval via the microwave method before IHC staining (13min in 10 mM citrate buffer pH6 followed by rapid cooling on ice). Immunoprecipitation: 1 ul per 10cm tissue culture dish of transfected cells. Use 10 ul protein A agarose CL4B to precipitate immune complex. Immunogold Electron Microscopy: 1:100.
Synonyms :
GFP
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.