This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
SERPINB9 / PI9 Antibody (clone 7D8) LS-C44956
catalog :
LS-C44956
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7D8

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
44694
AntibodyName :
LS-C44956
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
SERPINB9 / PI9 Antibody (clone 7D8) LS-C44956
Specificity :
Specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone Detects a band of approximately 42kD under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence.
ClonalityDesc :
Monoclonal
CloneName :
7D8
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.05% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant PI-9 produced in P. pastoris.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
SERPINB9 / PI9
StandardGeneSymbol :
SERPINB9
Reactivity :
Human
NonReactiveSpecies :
Mouse, Pig
Usage :
Flo (1:10 - 1:100), IHC, IHC-P (1:50), WB
ShortWebDescription :
PI9 antibody LS-C44956 is an unconjugated mouse monoclonal antibody to human PI9 (SERPINB9). Validated for Flow, IHC and WB.
UsageText :
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil.
Synonyms :
SERPINB9, CAP-3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, Serpin B9, PI-9, PI9
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.