This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD45 / LCA Antibody (clone UM16-6, RPE) LS-C44825
catalog :
LS-C44825
clonality :
monoclonal
host :
mouse
conjugate :
RPE
clone name :
UM16-6

The same clone is also sold as:
reactivity :
human, chicken
application :
flow cytometry
product information
AntibodyID :
44563
AntibodyName :
LS-C44825
TargetSpecies :
Chicken
Host Species :
Mouse
Product Name :
CD45 / LCA Antibody (clone UM16-6, RPE) LS-C44825
Specificity :
Recognizes chicken CD45 (leucocyte common antigen), a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of hematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the hematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. Studies have indicated that dephosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Investigations into the properties of CD45 in chicken models are limited, but there is evidence of the existence of an additional cysteine residue near the transmembrane region. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.
ClonalityDesc :
Monoclonal
CloneName :
UM16-6
AntibodyModification :
RPE
PresentationDesc :
PBS, 0.09% Sodium Azide, 1% BSA
ImmunogenDesc :
COS cells transfected with CD45 gene.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C. Do not freeze. Protect from light. Microcentrifugation recommended if solution contains precipitate.
ResuspensionLiquidDesc :
Reconstitute with distilled water.
IsotypeName :
IgG2a
Gene :
CD45 / LCA
StandardGeneSymbol :
PTPRC
gene family :
Protein Phosphatase
Subfamily :
R1/R6
Reactivity :
Chicken
Usage :
Flo (1:1 - 1:5)
ShortWebDescription :
LCA antibody LS-C44825 is an RPE-conjugated mouse monoclonal antibody to chicken LCA (CD45). Validated for Flow.
UsageText :
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.