This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CTLA4 / CD152 Antibody (clone BNI3) LS-C43849
catalog :
LS-C43849
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BNI3

The same clone is also sold as:
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
product information
AntibodyID :
43587
AntibodyName :
LS-C43849
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CTLA4 / CD152 Antibody (clone BNI3) LS-C43849
Specificity :
Recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single domain in the extracellular region. CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86. Is able to block ligand binding on the Raji B-cell line and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Further studies indicate that clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98,. The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2. CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface. CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases.
ClonalityDesc :
Monoclonal
CloneName :
BNI3
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide, 0.5% BSA
ImmunogenType :
Fusion protein
ImmunogenDesc :
Human CTLA-4/human IgG heavy chain fusion protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG2a
Gene :
CTLA4 / CD152
StandardGeneSymbol :
CTLA4
Reactivity :
Human
Usage :
Flo (1:1 - 1:10), IF, IHC, IHC-Fr (5 - 50 µg/ml), IP
UsageNotRecommended :
IHC-P
ShortWebDescription :
CD152 antibody LS-C43849 is an unconjugated mouse monoclonal antibody to human CD152 (CTLA4). Validated for Flow, IF, IHC and IP.
UsageText :
Flow Cytometry: Membrane permeabilization is required for this application. Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Synonyms :
CTLA4, CD152, CELIAC3, CD152 antigen, CTLA-4, Celiac disease 3, IDDM12, GRD4, GSE, CD152 isoform
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.