antibody

CD45 / LCA Antibody (clone YW62.3, RPE) LS-C43235

LS-C43235

monoclonal

rat

RPE

YW62.3

The same clone is also sold as:

• Bio-Rad: MCA1031SBV515, MCA1031A700T, MCA1031G, MCA1031SBUV665, MCA1031SBV440, MCA1031GT, MCA1031SBB700, MCA1031PE, MCA1031SBV610, MCA1031SBV475, MCA1031A488, MCA1031FB, MCA1031SBV670, MCA1031SBUV400, MCA1031SBUV510, MCA1031GA, MCA1031F, MCA1031AMO, MCA1031A700, MCA1031A647, MCA1031A647T, MCA1031A488T, MCA1031SBUV795
• OriGene: SM032PT
• Invitrogen: MA1-81247, MA1-80090
• Novus Biologicals: NB100-65366

human, mouse

LS-C43235

Mouse

Rat

CD45 / LCA Antibody (clone YW62.3, RPE) LS-C43235

Recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kD glycoprotein expressed by all leucocytes. CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains, this was subsequently expanded to include a third allele encoding Ly5.3. Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1. Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb;. A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. Is reactive with all isoforms of murine CD45. N. B. Some reactivity with human tissue has been observed.

Monoclonal

YW62.3

RPE

PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA

Cells

Mouse spleen cells.

Protein G purified

Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.

Reconstitute with distilled water.

IgG2b

CD45 / LCA

PTPRC

Protein Phosphatase

R1/R6

Mouse

Flo (1:1)

LCA antibody LS-C43235 is an RPE-conjugated rat monoclonal antibody to mouse LCA (CD45). Validated for Flow.

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA

Worldwide