antibody

YWHAZ / 14-3-3 Zeta Antibody (N-Terminus, Serum) LS-C42377

LS-C42377

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA, immunohistochemistry - paraffin section

LS-C42377

Sheep

Rabbit

YWHAZ / 14-3-3 Zeta Antibody (N-Terminus, Serum) LS-C42377

Recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30 kD proteins involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impaired Alzheimer's patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the Golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3 zeta can be phosphorylated at serine 184 to produce 14-3-3 delta. May not react with recombinant proteins that are not N-acetylated.

Polyclonal

Serum Unconjugated

N-Terminus

0.09% Sodium Azide

Synthetic peptide

Synthetic peptide corresponding to acetylated N-Terminus sequence of sheep 14-3-3 zeta.

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

IgG

YWHAZ / 14-3-3 Zeta

YWHAZ

Phospholipase

Sheep

ELISA, IHC-P (1:400), WB (1:3000)

14-3-3 Zeta antibody LS-C42377 is an unconjugated rabbit polyclonal antibody to sheep 14-3-3 Zeta (YWHAZ ). Validated for ELISA, IHC and WB.

Western Blot: LS-C42377 detects a band of approximately 30kD in HEK293 cell lysates. Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

YWHAZ, 14-3-3 protein zeta/delta, 14-3-3-zeta, 14-3-3 delta, 14-3-3 protein zeta, 14-3-3 zeta, Phospholipase A2, YWHAD, KCIP-1

Worldwide