This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NES / Nestin Antibody (clone Rat-401 (4D4)) LS-C42054
catalog :
LS-C42054
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Rat-401 (4D4)
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
product information
AntibodyID :
41792
AntibodyName :
LS-C42054
TargetSpecies :
Rat
Host Species :
Mouse
Product Name :
NES / Nestin Antibody (clone Rat-401 (4D4)) LS-C42054
Specificity :
Recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glial cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.
ClonalityDesc :
Monoclonal
CloneName :
Rat-401 (4D4)
AntibodyModification :
Unconjugated
PresentationDesc :
TBS, 0.09% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Nestin purified from embryonic rat spinal cord.
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
NES / Nestin
StandardGeneSymbol :
NES
Reactivity :
Rat
NonReactiveSpecies :
Human
Usage :
IF, IHC, IHC-Fr (1:20 - 1:200), WB
UsageNotRecommended :
IHC-P
ShortWebDescription :
Nestin antibody LS-C42054 is an unconjugated mouse monoclonal antibody to rat Nestin (NES). Validated for IF, IHC and WB.
UsageText :
Immunohistochemistry: We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. Western Blot: LS-C42054 reacts with a band at 200-220 kD in reducing gels of newborn rat or mouse cell extracts. For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Synonyms :
NES, Nestin, Nbla00170
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.