product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) LS-C41114
catalog :
LS-C41114
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
2F3
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 5
Reference
Loidl J, Mochizuki K. Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Mol Biol Cell. 2009;20:2428-37 pubmed publisher
Akbay E, Contreras C, Perera S, Sullivan J, Broaddus R, Schorge J, et al. Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Am J Pathol. 2008;173:536-44 pubmed publisher
Mochizuki K, Novatchkova M, Loidl J. DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. J Cell Sci. 2008;121:2148-58 pubmed publisher
Rossi D, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman I. Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Nature. 2007;447:725-9 pubmed
Xiao R, Sun Y, Ding J, Lin S, Rose D, Rosenfeld M, et al. Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Mol Cell Biol. 2007;27:5393-402 pubmed
image
image 1 :
LifeSpan Biosciences LS-C41114 image 1
Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.
image 2 :
LifeSpan Biosciences LS-C41114 image 2
Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
product information
AntibodyID :
40816
AntibodyName :
LS-C41114
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) LS-C41114
Specificity :
Modified peptide (KATQAS*QEY)
ClonalityDesc :
Monoclonal
CloneName :
2F3
AntibodyModification :
Unconjugated
AntigenModification :
pSer139
PresentationDesc :
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from human H2AFX / H2AX.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C. Store undiluted.
IsotypeName :
IgG2b
Gene :
H2AFX / H2AX
StandardGeneSymbol :
H2AFX
Reactivity :
Human
Usage :
ICC, IF, WB
ShortWebDescription :
H2AX antibody LS-C41114 is an unconjugated mouse monoclonal antibody to human H2AX (H2AFX) (pSer139). Validated for ICC, IF and WB. Cited in 5 publications.
Synonyms :
H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.