This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
WNT2B Antibody LS-C40260
catalog :
LS-C40260
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
39768
AntibodyName :
LS-C40260
TargetSpecies :
Mouse
Host Species :
Goat
Product Name :
WNT2B Antibody LS-C40260
Specificity :
Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized
ImmunogenDesc :
E. coli-derived rmWnt-2b.
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
WNT2B
StandardGeneSymbol :
WNT2B
gene family :
WNT
Reactivity :
Mouse, Human
Usage :
ELISA, ICC, WB
ShortWebDescription :
WNT2B antibody LS-C40260 is an unconjugated goat polyclonal antibody to WNT2B from mouse. It is reactive with human and mouse. Validated for ELISA, ICC and WB.
UsageText :
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20% cross-reactivity with rhWnt-2, and less than 5% cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well.
Synonyms :
WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.