product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
Vimentin Antibody LS-C40195
catalog :
LS-C40195
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; rat
Yamana D, Shimizu T, Fan Y, Miura T, Nanashima N, Yamada T, et al. Decrease of hepatic stellate cells in rats with enhanced sensitivity to clofibrate-induced hepatocarcinogenesis. Cancer Sci. 2011;102:735-41 pubmed publisher
product information
AntibodyID :
39703
AntibodyName :
LS-C40195
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Vimentin Antibody LS-C40195
Specificity :
Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.2, 0.1% Sodium Azide, No stabilizing proteins added
ImmunogenType :
Purified protein
ImmunogenDesc :
Vimentin
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
Vimentin
StandardGeneSymbol :
VIM
Reactivity :
Chicken, Rat, Human
Usage :
Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)
ShortWebDescription :
Vimentin antibody LS-C40195 is an unconjugated mouse monoclonal antibody to Vimentin from human. It is reactive with human, rat and chicken. Validated for Flow, ICC, IHC and WB. Cited in 1 publication.
UsageText :
Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer, pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens.
Synonyms :
VIM, Vimentin, vimenin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.