This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Trypsin 1,2,3 (PRSS1,2,3) Antibody (aa16-247) LS-C39952
catalog :
LS-C39952
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
antigen modification :
acetylated
reactivity :
human
application :
western blot, ELISA, neutralization, immunoprecipitation
product information
AntibodyID :
39460
AntibodyName :
LS-C39952
TargetSpecies :
Human
Host Species :
Sheep
Product Name :
Trypsin 1,2,3 (PRSS1,2,3) Antibody (aa16-247) LS-C39952
Specificity :
Selected for its ability to recognize human Trypsin 1, human Trypsin 2, and human Trypsin 3 in direct ELISAs and Western Blots.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa16-247
PresentationDesc :
Lyophilized
ImmunogenDesc :
NS0-derived rhTrypsin 2 (aa16-247).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
Trypsin 1,2,3 (PRSS1,2,3)
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), IP, Neut, WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
Trypsin 1,2,3 (PRSS1,2,3) antibody LS-C39952 is an unconjugated sheep polyclonal antibody to human Trypsin 1,2,3 (PRSS1,2,3) (aa16-247). Validated for ELISA, IP, Neut and WB.
UsageText :
Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3. The detection limit for rhTrypsin 1/2/3 is approximately 1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Used to immunoprecipitate rhTrypsin 1/2/3 from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhTrypsin 2. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.3 ug/ml (IC50) under conditions in which the enzyme was present at 0.02 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26 kD) and the antibody (150 kD), IC50 was achieved at approximately 3:1 molar ratio of the antibody to the enzyme. The antibody did not inhibit the activity of rhTrypsin 1 and rhTrypsin 3 significantly. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3. The detection limit for rhTrypsin 1/2/3 is approximately 0.8 ng/well.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.