This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
SMAD1 Antibody (phospho-Ser463/Ser465) LS-C39124
catalog :
LS-C39124
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot
product information
AntibodyID :
38632
AntibodyName :
LS-C39124
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
SMAD1 Antibody (phospho-Ser463/Ser465) LS-C39124
Specificity :
Recognizes the dual serine phosphorylated human Smad1 (Ser 463/465), which in the phosphorylated state has an apparent molecular weight of approximately 60-65kD. Species cross-reactivity: Mouse (Predicted cross-reactivity with human, rat and Xenopus).
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer463/Ser465
PresentationDesc :
0.1 M Tris-glycine, pH 7.2, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide (CG-GSPHNPIS[pS]V[pS]-GG) corresponding to amino acids 455-465 of human Smad1 protein (KLH).
PurificationDesc :
Protein A purified
IsotypeName :
IgG
Gene :
SMAD1
StandardGeneSymbol :
SMAD1
Reactivity :
Xenopus, Rat, Mouse, Human
Usage :
WB (0.5 - 2 µg/ml)
ShortWebDescription :
SMAD1 antibody LS-C39124 is an unconjugated rabbit polyclonal antibody to SMAD1 (pSer463/Ser465) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
UsageText :
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detected phosphorylated Smad1 in RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4. P19 cell lysate treated with BMP4 was resolved by electrophoresis, transferred to nitrocellulose and probed with phospho-Smad1 (Ser463/465) (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Note: A non-specific band was detected in P19 cell lysate, Mr 34-35kD.
Synonyms :
SMAD1, BSP1, BSP-1, JV4-1, MADH1, Mothers against DPP homolog 1, JV41, SMAD family member 1, TGF-beta signaling protein 1, MADR1, HSMAD1, MAD homolog 1, Mad-related protein 1, SMAD 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.