This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
REN / Renin 1 Antibody (aa24-406) LS-C38782
catalog :
LS-C38782
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, neutralization, immunoprecipitation
product information
AntibodyID :
38290
AntibodyName :
LS-C38782
TargetSpecies :
Human
Host Species :
Sheep
Product Name :
REN / Renin 1 Antibody (aa24-406) LS-C38782
Specificity :
Recognizes both the pro and mature forms of human Renin. In direct ELISAs and Western Blots, this antibody shows ~20% cross-reactivity with rmRenin-1. Species sequence homology: mouse 70%, canine 72%.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa24-406
PresentationDesc :
Lyophilized
ImmunogenDesc :
NS0-derived rhRenin (aa24-406).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
REN / Renin 1
StandardGeneSymbol :
REN
gene family :
Protease
Subfamily :
Aspartic A1
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), IHC (3 - 10 µg/ml), IP, Neut, WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
Renin 1 antibody LS-C38782 is an unconjugated sheep polyclonal antibody to human Renin 1 (REN) (aa24-406). Validated for ELISA, IHC, IP, Neut and WB.
UsageText :
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Neutralization. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 3-10 ug/ml. Immunoprecipitation: Used to immunoprecipitate rhRenin from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhRenin. The antibody was preincubated with the activated enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 40 ug/ml (IC50) under conditions in which the enzyme was present at 2 ug/ml and the substrate concentration was 5uM. Considering the molecular masses of the enzyme (44kD) and the antibody (150 kD), IC50 was achieved at approximately 6:1 molar ratio of the antibody to the enzyme. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 3 ng/well.
Synonyms :
REN, Angiotensin-forming enzyme, Angiotensinogenase, HNFJ2, RENAA, Renin precursor, renal, Renin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.