This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
HRAS / H-Ras Antibody (aa62-76) LS-C38766
catalog :
LS-C38766
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
38274
AntibodyName :
LS-C38766
TargetSpecies :
Human
Host Species :
Rat
Product Name :
HRAS / H-Ras Antibody (aa62-76) LS-C38766
Specificity :
Recognizes phosphorylated and non-phosphorylated human, rat and mouse ras. Does not cross-react with the rap protein. Neutralizes the biological and biochemical activities by binding to residues E63, S65, A66, M67, Q70 and R73. Positive Controls: SW480 or Y1 cells (IF and IP); normal skin (IHC, frozen and paraffin); A431, Hela or ML-1 cells (WB).
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa62-76
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant p21 ras protein. Epitope: aa62-76 of human ras.
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
HRAS / H-Ras
StandardGeneSymbol :
HRAS
gene family :
Ras GTPase superfamily IPR001806
Subfamily :
RAS oncogene
Reactivity :
Mouse, Rat, Human
Usage :
IF (2.5 µg/ml), IHC, IHC-Fr, IHC-P, IP, Neut, WB (2.5 µg/ml)
ShortWebDescription :
H-Ras antibody LS-C38766 is an unconjugated rat monoclonal antibody to H-Ras (HRAS) (aa62-76) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP, Neut and WB.
UsageText :
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Immunofluorescence: 2.5 ug/ml. Western Blot (chemiluminescence detection): 2.5 ug/ml. Immunohistochemistry (Frozen): 5 ug/ml. Immunohistochemistry: 1-5 ug/ml. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation (autoradiographic detection): 1 ug/sample SW480 or Y1 cells. Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes.
Synonyms :
HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21ras, RASH1, C-BAS/HAS, C-ha-ras, p19 H-RasIDX protein
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.