This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CCL5 / RANTES Antibody LS-C38737
catalog :
LS-C38737
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, neutralization
product information
AntibodyID :
38245
AntibodyName :
LS-C38737
TargetSpecies :
Cat
Host Species :
Sheep
Product Name :
CCL5 / RANTES Antibody LS-C38737
Specificity :
Selected for its ability to neutralize feline RANTES bioactivity.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized
ImmunogenDesc :
E. coli-derived rfeRANTES.
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
CCL5 / RANTES
StandardGeneSymbol :
CCL5
gene family :
Intercrine
Reactivity :
Cat
Usage :
ELISA (0.5 - 1 µg/ml), Func, Neut, WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
RANTES antibody LS-C38737 is an unconjugated sheep polyclonal antibody to cat RANTES (CCL5). Validated for ELISA, Func, Neut and WB.
UsageText :
Suitable for use in ELISA, Western Blot, Neutralization. Neutralization of Feline CCL5 Bioactivity: The exact concentration of antibody required to neutralize rfeRANTES activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-feline RANTES antibody was determined to be approximately 0.5-2.5 ug/ml in the presence of 80 ng/mL of rfeRANTES, using transfected BaF/3 cells in a chemotaxis assay. The specific conditions are described in the figure legends. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 1 ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 0.5 ng/well. In this format, this antibody shows less than 5% cross-reactivity with rcrRANTES, rhRANTES, and rmRANTES.
Synonyms :
CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.