This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PRKCB / PKC-Beta Antibody (aa607-671) LS-C38534
catalog :
LS-C38534
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
38042
AntibodyName :
LS-C38534
TargetSpecies :
Human
Host Species :
Goat
Product Name :
PRKCB / PKC-Beta Antibody (aa607-671) LS-C38534
Specificity :
Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa607-671
PresentationDesc :
Lyophilized
ImmunogenDesc :
E. coli-derived rhPKCb1 (aa 607-671).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
PRKCB / PKC-Beta
StandardGeneSymbol :
PRKCB
gene family :
Protein Kinase
Subfamily :
PKC
Reactivity :
Rat, Human
Usage :
ELISA, ICC, WB
ShortWebDescription :
PKC-Beta antibody LS-C38534 is an unconjugated goat polyclonal antibody to PKC-Beta (PRKCB) (aa607-671) from human. It is reactive with human and rat. Validated for ELISA, ICC and WB.
UsageText :
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1% cross-reactivity with rhPKC.
Synonyms :
PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.