This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NOTCH4 Antibody (aa1778-2003) LS-C37776
catalog :
LS-C37776
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
37284
AntibodyName :
LS-C37776
TargetSpecies :
Human
Host Species :
Goat
Product Name :
NOTCH4 Antibody (aa1778-2003) LS-C37776
Specificity :
Recognizes human Notch4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1778-2003
PresentationDesc :
Lyophilized
ImmunogenDesc :
E. coli-derived rhNotch-4 (aa 1778-2003).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
NOTCH4
StandardGeneSymbol :
NOTCH4
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), ICC (10 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
NOTCH4 antibody LS-C37776 is an unconjugated goat polyclonal antibody to human NOTCH4 (aa1778-2003). Validated for ELISA, ICC and WB.
UsageText :
Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well.
Synonyms :
NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.