This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PNOC / Nociceptin Antibody (aa122-138) LS-C37733
catalog :
LS-C37733
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
immunohistochemistry, immunocytochemistry
product information
AntibodyID :
37241
AntibodyName :
LS-C37733
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
PNOC / Nociceptin Antibody (aa122-138) LS-C37733
Specificity :
Species Reactivity: Rat and Primate.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa122-138
PresentationDesc :
0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to residues 122-138 of rat Orphanin FQ.
PurificationDesc :
Antiserum
IsotypeName :
IgG
Gene :
PNOC / Nociceptin
StandardGeneSymbol :
PNOC
gene family :
Endogenous opioids
Reactivity :
Rat
Usage :
IF, IHC (1:500)
ShortWebDescription :
Nociceptin antibody LS-C37733 is an unconjugated rabbit polyclonal antibody to rat Nociceptin (PNOC) (aa122-138). Validated for IF and IHC.
UsageText :
Suitable for use in Immunohistochemistry. Immunohistochemistry: 1:500. This orphanin FQ robustly stains nerve fibers in brain and spinal cord. Animals were treated with colchicines to visualize orphanin FQ-expressing cell bodies. Staining was blocked by preabsorption with the cognate peptide. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
Synonyms :
PNOC, Nociceptin, Nocistatin, Propronociceptin, OFQ, PPNOC, Prepronociceptin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.