This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CHRM3 / M3 Antibody (C-Terminus) LS-C37395
catalog :
LS-C37395
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
36903
AntibodyName :
LS-C37395
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
CHRM3 / M3 Antibody (C-Terminus) LS-C37395
Specificity :
Recognizes rat M3 Muscarinic Receptor. Does not recognize M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M4 Muscarinic Receptor (469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. Specific for the C-terminal of the M3 receptor.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
Lyophilized
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Peptide analog of the C-Terminus of muscarinic M3 receptor covalently attached onto a carrier protein.
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
100ul PBS, 10mg/ml BSA
Gene :
CHRM3 / M3
StandardGeneSymbol :
CHRM3
gene family :
GPCR
Subfamily :
Acetylcholine
Reactivity :
Human
Usage :
ELISA, IHC, WB (1:500)
ShortWebDescription :
M3 antibody LS-C37395 is an unconjugated rabbit polyclonal antibody to human M3 (CHRM3) (C-Terminus). Validated for ELISA, IHC and WB.
UsageText :
Western Blot: 1:500Immunohistochemistry: 1:2500 using PLP-fixed rat brain sections (ABC technique)Optimal dilutions to be determined by the researcher.Western Blot Protocol:1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels, such at 12% gels) and electrophoretic transfer to PVDFmembrane, block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.2. Wash 2X with TBS/Tween-20.3. Apply LS-C37395 at dilution of at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 asbuffer for the primary antibody. Incubate for 2-4 hours.4. Wash 3X with TBS/Tween-20.5. Apply affinity purified IgG (HRP) goat x rabbit antiserum (dilution may vary depending upon supplier) in 2% normal goat serum inTBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash 4X for 5 minute/wash with TBS/Tween-20.7. Develop color using the enhanced DAB reaction.
Synonyms :
CHRM3, M3, Muscarinic receptor M3, Muscarinic M3 receptor, EGBRS, HM3, M3 muscarinic receptor
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.