This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Latexin / MUM Antibody (aa2-222) LS-C37156
catalog :
LS-C37156
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, neutralization, immunoprecipitation
product information
AntibodyID :
36664
AntibodyName :
LS-C37156
TargetSpecies :
Mouse
Host Species :
Sheep
Product Name :
Latexin / MUM Antibody (aa2-222) LS-C37156
Specificity :
Selected for its ability to recognize mouse Latexin in direct ELISAs and Western Blots. In these formats, this antibody shows ~40% cross-reactivity with rhLatexin.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa2-222
PresentationDesc :
Lyophilized
ImmunogenDesc :
E. coli-derived rmLatexin (aa 2-222).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
Latexin / MUM
StandardGeneSymbol :
LXN
Reactivity :
Mouse
Usage :
ELISA, IP, Neut, WB
ShortWebDescription :
MUM antibody LS-C37156 is an unconjugated sheep polyclonal antibody to mouse MUM (Latexin) (aa2-222). Validated for ELISA, IP, Neut and WB.
UsageText :
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells. Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50% of the proteolytic activity was restored by the antibody at 4.4 ug/ml (IC50) under conditions in which 0.1 ug/ml of the inhibitor, 0.1 ug/ml of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 molar ratio of the antibody to the inhibitor. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well.
Synonyms :
LXN, ECI, MUM, Protein MUM, TCI, Latexin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.