This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
KLK13 / Kallikrein 13 Antibody (aa19-262) LS-C37112
catalog :
LS-C37112
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, neutralization, immunoprecipitation
product information
AntibodyID :
36620
AntibodyName :
LS-C37112
TargetSpecies :
Human
Host Species :
Goat
Product Name :
KLK13 / Kallikrein 13 Antibody (aa19-262) LS-C37112
Specificity :
Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10% cross-reactivity with rhKallikrein 8, and less than 5% cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa19-262
PresentationDesc :
Lyophilized
ImmunogenDesc :
NS0-derived rhKLK13 (aa19-262).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
KLK13 / Kallikrein 13
StandardGeneSymbol :
KLK13
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Human
Usage :
ELISA, IP, Neut, WB
ShortWebDescription :
Kallikrein 13 antibody LS-C37112 is an unconjugated goat polyclonal antibody to human Kallikrein 13 (KLK13) (aa19-262). Validated for ELISA, IP, Neut and WB.
UsageText :
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50% of the proteolytic activity was inhibited by the antibody at approximately 8.6 ug/ml (IC50) under conditions in which the enzyme was present at 1 ug/ml and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well.
Synonyms :
KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.