CARMA1 / CARD11 Antibody (N-Terminus) LS-C368868 | LifeSpan Biosciences LS-C368868 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

CARMA1 / CARD11 Antibody (N-Terminus) LS-C368868

catalog :

LS-C368868

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Dorjbal B, Stinson J, Ma C, Weinreich M, Miraghazadeh B, Hartberger J, et al. Hypomorphic caspase activation and recruitment domain 11 (CARD11) mutations associated with diverse immunologic phenotypes with or without atopic disease. J Allergy Clin Immunol. 2019;143:1482-1495 pubmed publisher

image

image 1 :

Western blot analysis of CARD11 expression in K562 (A); Ramos (B); Jurkat (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of CARD11 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of CARD11 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

AntibodyID :

380927

AntibodyName :

LS-C368868

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

CARMA1 / CARD11 Antibody (N-Terminus) LS-C368868

Specificity :

Recognizes endogenous levels of CARD11 protein.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

N-Terminus

PresentationDesc :

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

ImmunogenType :

Synthetic peptide - KLH conjugated

ImmunogenDesc :

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human CARD11.

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Gene :

CARMA1 / CARD11

BlockingPeptide :

LS-E43739

StandardGeneSymbol :

CARD11

gene family :

Apoptosis

Reactivity :

Mouse, Human

Usage :

ICC (1:100 - 1:500), IF, IHC, IHC-P (1:100 - 1:200), WB (1:500 - 1:1000)

ShortWebDescription :

CARD11 antibody LS-C368868 is an unconjugated rabbit polyclonal antibody to CARD11 (CARMA1) (N-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB. Cited in 2 publications.

Synonyms :

CARD11, Card-maguk protein 1, CARMA1, Carma 1, BIMP3

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.