product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
SOX9 Antibody (Internal) LS-C368527
catalog :
LS-C368527
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 1
image
image 1 :
Western blot analysis of SOX9 expression in HepG2 (A); HeLa (B); NIH3T3 (C) whole cell lysates.
image 2 :
Immunohistochemical analysis of SOX9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of SOX9 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
AntibodyID :
380586
AntibodyName :
LS-C368527
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
SOX9 Antibody (Internal) LS-C368527
Specificity :
Recognizes endogenous levels of SOX9 protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human SOX9.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Gene :
SOX9
BlockingPeptide :
LS-E42497
StandardGeneSymbol :
SOX9
gene family :
Transcription factor
Reactivity :
Mouse, Dog, Rat, Pig, Human
Usage :
Flo, ICC (1:100 - 1:500), IF, IHC, IHC-P (1:100 - 1:200), IP (1:10 - 1:100), WB (1:500 - 1:1000)
ShortWebDescription :
SOX9 antibody LS-C368527 is an unconjugated rabbit polyclonal antibody to SOX9 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, ICC, IF, IHC, IP and WB. Cited in 1 publication.
Synonyms :
SOX9, CMD1, CMPD1, SRY-related HMG-box, gene 9, Transcription factor SOX-9
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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