antibody

TERF2 / TRF2 Antibody (clone 4A794.15, Azide-free) LS-C368271

LS-C368271

monoclonal

mouse

nonconjugated

4A794.15

The same clone is also sold as:

• Rockland Immunochemicals: 200-301-I34
• Abnova: MAB0074
• OriGene: TA336371
• Novus Biologicals: NBP2-33212, NB100-56506
• Invitrogen: MA1-41001

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

LS-C368271

Human

Mouse

TERF2 / TRF2 Antibody (clone 4A794.15, Azide-free) LS-C368271

Human TERF2 / TRF2. The recognizes distinct doublet bands of approximate size 66 kD corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines.

Monoclonal

4A794.15

Azide-free Unconjugated

PBS. No stabilizing proteins added.

Cells

Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).

Protein G purified

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG1,k

TERF2 / TRF2

TERF2

Mouse, Rat, Human

ChrIP (1:10 - 1:500), ELISA (2 µg/ml), Flo (0.1 µg/10E6 cells), ICC (1:10 - 1:500), IF (1:10 - 1:500), IHC, IHC-Fr (5 µg/ml), IHC-P (1:10 - 1:500), IP (2 µg/10E6 cells), WB (2 - 4 µg/ml)

TRF2 antibody LS-C368271 is an unconjugated mouse monoclonal antibody to TRF2 (TERF2) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ELISA, Flow, ICC, IF, IHC, IP and WB.

TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

TERF2, TRF2, TRBF2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

Worldwide