This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
GLI3 Antibody LS-C36438
catalog :
LS-C36438
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
35946
AntibodyName :
LS-C36438
TargetSpecies :
Human
Host Species :
Goat
Product Name :
GLI3 Antibody LS-C36438
Specificity :
Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1% cross-reactivity with rhGLI-1 and rhGLI-2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized
ImmunogenDesc :
E. coli-derived rhGLI-3.
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG
Gene :
GLI3
StandardGeneSymbol :
GLI3
Reactivity :
Mouse, Human
Usage :
ELISA (0.5 - 1 µg/ml), ICC (10 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
GLI3 antibody LS-C36438 is an unconjugated goat polyclonal antibody to GLI3 from human. It is reactive with human and mouse. Validated for ELISA, ICC and WB.
UsageText :
Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 ug/ml. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 ug/ml detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 ug/ml. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well.
Synonyms :
GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.