This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
AKT3 Antibody (aa119-131) LS-C3627
catalog :
LS-C3627
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunoprecipitation
product information
AntibodyID :
4368
AntibodyName :
LS-C3627
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
AKT3 Antibody (aa119-131) LS-C3627
Specificity :
Recognizes Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species cross-reactivity: Human. Species sequence homology: mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa119-131
PresentationDesc :
0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%.
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated, synthetic peptide corresponding to aa119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (92%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
AKT3
StandardGeneSymbol :
AKT3
gene family :
Protein Kinase
Subfamily :
RAC/AKT
Reactivity :
Chicken, Bovine, Rat, Pig, Horse, Bat, Rabbit, Mouse, Dog, Human, Monkey
Usage :
Func, IP, WB (1:500 - 1:2000)
ShortWebDescription :
AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 (aa119-131) from human. It is reactive with human, mouse, rat and other species. Validated for Func, IP and WB.
UsageText :
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Immunoprecipitation: 5 ul immunoprecipitated active Akt3/PKBg from 15 nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg).
Synonyms :
AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.