This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
DLL1 Antibody (Extracellular Domain) LS-C36154
catalog :
LS-C36154
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
35662
AntibodyName :
LS-C36154
TargetSpecies :
Rat
Host Species :
Sheep
Product Name :
DLL1 Antibody (Extracellular Domain) LS-C36154
Specificity :
Recognize rat DLL1. Species sequence homology: mouse (97%), human (91%), over aa18-537.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Extracellular Domain
PresentationDesc :
Lyophilized from Sterile Solution PBS, 5% Trehalose
ImmunogenDesc :
NS0-derived rrDLL1 extracellular domain.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
40-50% glycerol, PBS
IsotypeName :
IgG
Gene :
DLL1
StandardGeneSymbol :
DLL1
Reactivity :
Rat
Usage :
ELISA (0.5 - 1 µg/ml), IHC (10 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
DLL1 antibody LS-C36154 is an unconjugated sheep polyclonal antibody to rat DLL1 (Extracellular Domain). Validated for ELISA, IHC and WB.
UsageText :
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 ug/ml Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 ug/ml Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4%PFA+14%picric acid in PBS at 4? C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: 0.5-1.0 ug/ml Detection limit: 0.3 ng/well.~10% cross-reactivity with rhDLL-1 and >1% cross reactivity with rrNotch-1.
Synonyms :
DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.