This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CHAT Antibody (clone 1.B3.9B3) LS-C35920
catalog :
LS-C35920
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1.B3.9B3
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
product information
AntibodyID :
35428
AntibodyName :
LS-C35920
TargetSpecies :
Pig
Host Species :
Mouse
Product Name :
CHAT Antibody (clone 1.B3.9B3) LS-C35920
Specificity :
Recognizes choline acetyltransferase (ChAT)-positive cells. Species cross-reactivity: Human, Rat and Porcine.
ClonalityDesc :
Monoclonal
CloneName :
1.B3.9B3
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.6, 0.09% Sodium Azide
ImmunogenDesc :
Highly purified ChAT obtained from porcine brain
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
CHAT
StandardGeneSymbol :
CHAT
Reactivity :
Rat, Pig, Human
Usage :
ELISA, IHC, IHC-Pfa (10 - 20 µg/ml), WB
ShortWebDescription :
CHAT antibody LS-C35920 is an unconjugated mouse monoclonal antibody to CHAT from pig. It is reactive with human, rat and pig. Validated for ELISA, IHC and WB.
UsageText :
Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4% paraformaldehyde/10% formaldehyde for 1 week at 4°C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10%, 20% and 30%). Prepare 50um sections and store in a cryoprotective solution at -15°C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections.
Synonyms :
CHAT, Choline acetylase, Choline acetyltransferase, Choline O-acetyltransferase, CMS1A2, CHOACTASE, CMS1A
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.