This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CHIT1 / Chitotriosidase Antibody LS-C35914
catalog :
LS-C35914
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
neutralization, immunoprecipitation
product information
AntibodyID :
35422
AntibodyName :
LS-C35914
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CHIT1 / Chitotriosidase Antibody LS-C35914
Specificity :
Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhCHI-3L1. Species sequence homology: mouse: 79%.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized
ImmunogenDesc :
NS0-derived rhCHIT-1.
PurificationDesc :
Protein G purified
ResuspensionLiquidDesc :
Reconstitute in deionized water
IsotypeName :
IgG1
Gene :
CHIT1 / Chitotriosidase
StandardGeneSymbol :
CHIT1
Reactivity :
Mouse, Human
Usage :
ELISA, IP (25 µg/ml), Neut
ShortWebDescription :
Chitotriosidase antibody LS-C35914 is an unconjugated mouse monoclonal antibody to Chitotriosidase (CHIT1) from human. It is reactive with human and mouse. Validated for ELISA, IP and Neut.
UsageText :
Suitable for use in ELISA, Immunoprecipitation and Neutralization. ELISA (direct): 0.5-1.0 ug/ml. Detection limit for rhCHIT1 is approximately 2 ng/well. Immunoprecipitation: 25 ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N, N', N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56 ng/mL (IC 50) under conditions in which the enzyme was present at 20 ng/mL and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.
Synonyms :
CHIT1, CHITD, CHI3, Chitinase-1, Chitinase 1 (chitotriosidase), Chitotriosidase, CHIT, Chitotriosidase-1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.