This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
HIF1A / HIF1 Alpha Antibody (clone HIF1A-84) LS-C357910
catalog :
LS-C357910
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HIF1A-84

The same clone is also sold as:
reactivity :
human
application :
immunocytochemistry, flow cytometry
product information
AntibodyID :
369455
AntibodyName :
LS-C357910
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
HIF1A / HIF1 Alpha Antibody (clone HIF1A-84) LS-C357910
Specificity :
Human HIF1A / HIF1 Alpha
ClonalityDesc :
Monoclonal
CloneName :
HIF1A-84
AntibodyModification :
Unconjugated
PresentationDesc :
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human HIF1 alpha.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 2°C to 8°C.
IsotypeName :
IgG2b,k
Gene :
HIF1A / HIF1 Alpha
StandardGeneSymbol :
HIF1A
Reactivity :
Human
Usage :
ELISA, Flo (0.5 - 1 µg/10E6 cells), IF (0.5 - 1 µg/ml)
ShortWebDescription :
HIF1 Alpha antibody LS-C357910 is an unconjugated mouse monoclonal antibody to human HIF1 Alpha (HIF1A). Validated for ELISA, Flow and IF.
UsageText :
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (alpha and beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex. ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIFs regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. ELISA: Order BSA-free antibody format for coating.
Synonyms :
HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.