This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD45R Antibody (clone RA3-3A1/6.1, Biotin) LS-C357692
catalog :
LS-C357692
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
RA3-3A1/6.1
application :
flow cytometry
product information
AntibodyID :
369214
AntibodyName :
LS-C357692
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
CD45R Antibody (clone RA3-3A1/6.1, Biotin) LS-C357692
Specificity :
Antibody RA3-3A1 binds to an extracellular epitope of B220 (CD45R), the 220 kD variant of the T200, CD45 molecule present on B cells and B cell precursors.
ClonalityDesc :
Monoclonal
CloneName :
RA3-3A1/6.1
AntibodyModification :
Biotin
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Cells
ImmunogenDesc :
RAW112 cells.
PurificationDesc :
Size exclusion chromatography
RecommendedStorageDesc :
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
IsotypeName :
IgM
Gene :
CD45R
Reactivity :
Mouse
Usage :
Flo
ShortWebDescription :
CD45R antibody LS-C357692 is a biotin-conjugated rat monoclonal antibody to mouse CD45R. Validated for Flow.
UsageText :
Reagent was tested for binding to ACK-lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-mouse B220/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with secondary reagent Streptavidin/PE after which they were washed three times, fixed and analyzed by FACS. A 33% sub population of the cells stained positive with a mean shift of 2.44 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse B220 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.