CD3E Antibody (clone 145-2C11) LS-C357691 | LifeSpan Biosciences LS-C357691 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

CD3E Antibody (clone 145-2C11) LS-C357691

catalog :

LS-C357691

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

145-2C11

reactivity :

human, mouse

application :

western blot, ELISA, immunoprecipitation, flow cytometry

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Rehrauer H, Wu L, Blum W, Pecze L, Henzi T, Serre Beinier V, et al. How asbestos drives the tissue towards tumors: YAP activation, macrophage and mesothelial precursor recruitment, RNA editing, and somatic mutations. Oncogene. 2018;37:2645-2659 pubmed publisher

image

image 1 :

Flow cytometry of CD3E antibody

product information

AntibodyID :

369213

AntibodyName :

LS-C357691

TargetSpecies :

Mouse

Host Species :

Hamster

Product Name :

CD3E Antibody (clone 145-2C11) LS-C357691

Specificity :

Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

ClonalityDesc :

Monoclonal

CloneName :

145-2C11

AntibodyModification :

Unconjugated

PresentationDesc :

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

ImmunogenType :

Membrane preparation

ImmunogenDesc :

Mouse BM10-37 T cell membrane prep.

PurificationDesc :

Protein G purified

RecommendedStorageDesc :

Store at 4°C, avoid freeze-thaw cycles.

IsotypeName :

IgG

Gene :

CD3E

StandardGeneSymbol :

CD3E

Reactivity :

Mouse

Usage :

ELISA, Flo, IP, WB

ShortWebDescription :

CD3E antibody LS-C357691 is an unconjugated hamster monoclonal antibody to mouse CD3E. Validated for ELISA, Flow, IP and WB.

UsageText :

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20ul of 300ug/ml murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD3 antibody diluted to 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33% sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background.

Synonyms :

CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon, CD3

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.