This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD3E Antibody (clone 145-2C11, FITC) LS-C357689
catalog :
LS-C357689
clonality :
monoclonal
host :
hamsters
conjugate :
FITC
clone name :
145-2C11

The same clone is also sold as:
reactivity :
human, mouse
application :
western blot, ELISA, immunoprecipitation, flow cytometry
product information
AntibodyID :
369211
AntibodyName :
LS-C357689
TargetSpecies :
Mouse
Host Species :
Hamster
Product Name :
CD3E Antibody (clone 145-2C11, FITC) LS-C357689
Specificity :
Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.
ClonalityDesc :
Monoclonal
CloneName :
145-2C11
AntibodyModification :
FITC
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Membrane preparation
ImmunogenDesc :
Mouse BM10-37 T cell membrane prep.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
IsotypeName :
IgG
Gene :
CD3E
StandardGeneSymbol :
CD3E
Reactivity :
Mouse
Usage :
ELISA, Flo, IP, WB
ShortWebDescription :
CD3E antibody LS-C357689 is an FITC-conjugated hamster monoclonal antibody to mouse CD3E. Validated for ELISA, Flow, IP and WB.
UsageText :
Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-mouse CD3/FITC at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 35% sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon, CD3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.