This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
EPCAM Antibody (clone ANC8D4, FITC) LS-C357671
catalog :
LS-C357671
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
ANC8D4
reactivity :
human
application :
flow cytometry
product information
AntibodyID :
369192
AntibodyName :
LS-C357671
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
EPCAM Antibody (clone ANC8D4, FITC) LS-C357671
Specificity :
Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.
ClonalityDesc :
Monoclonal
CloneName :
ANC8D4
AntibodyModification :
FITC
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human CD326(EpCAM).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
IsotypeName :
IgG1,k
Gene :
EPCAM
StandardGeneSymbol :
EPCAM
Reactivity :
Human
Usage :
ELISA, Flo
ShortWebDescription :
EPCAM antibody LS-C357671 is an FITC-conjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow.
UsageText :
Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of product at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.30 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.