This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CEACAM8 / CD66b Antibody (clone ANC3C5, Biotin) LS-C357669
catalog :
LS-C357669
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
ANC3C5
reactivity :
human
application :
flow cytometry
product information
AntibodyID :
369190
AntibodyName :
LS-C357669
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CEACAM8 / CD66b Antibody (clone ANC3C5, Biotin) LS-C357669
Specificity :
Antibody from clone ANC3C5 binds to CD66b on the cell surface of human PMN in Flow cytometry and works as an EIA capture or detection reagent for recombinant CD66b when paired with clone ANC4A4.
ClonalityDesc :
Monoclonal
CloneName :
ANC3C5
AntibodyModification :
Biotin
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant soluble human CD66b.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C, avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
CEACAM8 / CD66b
StandardGeneSymbol :
CEACAM8
gene family :
CEA
Reactivity :
Human
Usage :
ELISA, Flo
ShortWebDescription :
CD66b antibody LS-C357669 is a biotin-conjugated mouse monoclonal antibody to human CD66b (CEACAM8). Validated for ELISA and Flow.
UsageText :
Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD66b antibody(ANC3C5) at 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.66 log10 fluorescent units when compared to a Mouse IgG1/Biotin isotype control. Binding was blocked when cells were pre incubated with an excess of unlabeled anti-human CD66b (ANC3C5) mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
CEACAM8, CD66b antigen, Carcinoembryonic antigen CGM6, CD67, CD67 antigen, CGM6, NCA-95, CD66b
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.