This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NGFR / CD271 / TNR16 Antibody (clone ANC271/3D7, Biotin) LS-C357664
catalog :
LS-C357664
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
ANC271/3D7
reactivity :
human
application :
flow cytometry
product information
AntibodyID :
369185
AntibodyName :
LS-C357664
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
NGFR / CD271 / TNR16 Antibody (clone ANC271/3D7, Biotin) LS-C357664
Specificity :
Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.
ClonalityDesc :
Monoclonal
CloneName :
ANC271/3D7
AntibodyModification :
Biotin
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant soluble human CD271.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C, avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
NGFR / CD271 / TNR16
StandardGeneSymbol :
NGFR
gene family :
TNF Receptor
Reactivity :
Human
Usage :
ELISA, Flo
ShortWebDescription :
TNR16 antibody LS-C357664 is a biotin-conjugated mouse monoclonal antibody to human TNR16 (NGFR / CD271). Validated for ELISA and Flow.
UsageText :
Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD271/Biotin at a concentration of 10 ug/ml. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16, p75-NTR
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.