This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD8A / CD8 Alpha Antibody (clone 53-6.72, APC) LS-C357658
catalog :
LS-C357658
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
53-6.72

The same clone is also sold as:
reactivity :
human, mouse
application :
flow cytometry
product information
AntibodyID :
369179
AntibodyName :
LS-C357658
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
CD8A / CD8 Alpha Antibody (clone 53-6.72, APC) LS-C357658
Specificity :
Mouse CD8.
ClonalityDesc :
Monoclonal
CloneName :
53-6.72
AntibodyModification :
APC
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
ImmunogenType :
Cells
ImmunogenDesc :
Mouse thymocytes/splenocytes.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C. Do not freeze. Protect from light.
IsotypeName :
IgG2a,k
Gene :
CD8A / CD8 Alpha
StandardGeneSymbol :
CD8A
Reactivity :
Mouse
Usage :
ELISA, Flo
ShortWebDescription :
CD8 Alpha antibody LS-C357658 is an APC-conjugated rat monoclonal antibody to mouse CD8 Alpha (CD8A). Validated for ELISA and Flow.
UsageText :
Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD8/APC at a 1:50 dilution (2 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 9% sub population of the cells stained positive with a mean shift of 2.59 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD8 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.