This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PD-L2 / PDCD1LG2 / CD273 Antibody (clone ANC8D12, Biotin) LS-C357632
catalog :
LS-C357632
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
ANC8D12
reactivity :
human
application :
neutralization, flow cytometry
product information
AntibodyID :
369152
AntibodyName :
LS-C357632
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PD-L2 / PDCD1LG2 / CD273 Antibody (clone ANC8D12, Biotin) LS-C357632
Specificity :
Antibody ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.
ClonalityDesc :
Monoclonal
CloneName :
ANC8D12
AntibodyModification :
Biotin
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human CD273(B7-DC).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C, avoid freeze-thaw cycles.
IsotypeName :
IgG2a,k
Gene :
PD-L2 / PDCD1LG2 / CD273
StandardGeneSymbol :
PDCD1LG2
Reactivity :
Human
Usage :
Block, ELISA, Flo
ShortWebDescription :
CD273 antibody LS-C357632 is a biotin-conjugated mouse monoclonal antibody to human CD273 (PD-L2 / PDCD1LG2). Validated for Block, ELISA and Flow.
UsageText :
Human monocyte derived Dendritic cells were prepared in 8 day culture. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of antihuman CD273/Biotin at concentration of 10 ug/ml. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.