This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PVR / CD155 Antibody (clone ANC6A3, Biotin) LS-C357623
catalog :
LS-C357623
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
ANC6A3
reactivity :
human
application :
flow cytometry
product information
AntibodyID :
369142
AntibodyName :
LS-C357623
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PVR / CD155 Antibody (clone ANC6A3, Biotin) LS-C357623
Specificity :
Antibody ANC6A3 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.
ClonalityDesc :
Monoclonal
CloneName :
ANC6A3
AntibodyModification :
Biotin
PresentationDesc :
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant Human CD155.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C, avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
PVR / CD155
StandardGeneSymbol :
PVR
Reactivity :
Human
Usage :
ELISA, Flo
ShortWebDescription :
CD155 antibody LS-C357623 is a biotin-conjugated mouse monoclonal antibody to human CD155 (PVR). Validated for ELISA and Flow.
UsageText :
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 ^l of 300 ^g/ml human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD155/Biotin at concentration of 10 ^g/ml. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.48 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
PVR, CD155, Hpvr, HVED, Nectin-like 5, Nectin-like protein 5, Poliovirus receptors beta, Poliovirus receptor, PVS, Necl-5, CD155 antigen, NECL5, TAGE4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.